Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (2)
- (2)
- (1)
- (27)
- (5)
- (7)
- (4)
- (2)
- (6)
- (1)
- (1)
- (3)
- (3)
- (2)
- (3)
- (5)
- (2)
- (2)
- (7)
- (16)
- (21)
- (3)
- (2)
- (3)
- (5)
- (1)
- (2)
- (1)
- (1)
- (1)
- (1)
- (1)
- (1)
- (3)
- (1)
- (1)
- (2)
- (1)
Filtered Search Results
Gibco™ DPBS, no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS, no calcium, no magnesium
Dilution | 1 X |
---|---|
Content And Storage | 15°to 30°C |
Form | Liquid |
Product Type | DPBS |
Thermo Scientific Chemicals MES monohydrate, 99+%, for biochemistry
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 195.23 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid monohydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
PubChem CID | 16218417 |
---|---|
CAS | 145224-94-8 |
Molecular Weight (g/mol) | 195.23 |
MDL Number | MFCD00149409 |
SMILES | C1COCCN1CCS(=O)(=O)O.O |
Synonym | 2-(N-Morpholino)ethanesulfonic acid monohydrate |
IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
Molecular Formula | C6H15NO5S |
Gibco™ DPBS (10X), calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS (10X), calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Capacity | 500 mL |
pH | 4.3 to 4.7 |
Shelf Life | 24 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2700 to 3200 mOsm/kg |
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS (10X), no calcium, no magnesium
Dilution | 10 X |
---|---|
Content And Storage | 15°to 30°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 6 to 7 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Osmolality | 2500 to 2900 mOsm/kg |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
DPBS, calcium, magnesium
Regulatory Status | For in-vitro diagnostic use |
---|---|
Dilution | 1 X |
Content And Storage | 2° to 8°C |
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
pH | 7 to 7.2 |
Shelf Life | 36 Month(s) |
For Use With (Application) | Mammalian Cell Culture |
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Content And Storage | Room temperature |
---|---|
Form | Liquid |
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!
TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Invitrogen™ Tris (1 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 1M Tris, pH 8.0 solution is supplied in one bottle. The buffer is certified RNase-free, economical, and ready-to-use.
Formulation | 1,3-Propanediol, 2-Amino-2-(Hydroxymethyl)-,Hydrochloride |
---|---|
Physical Form | Liquid |
pH | 8 |
Grade | Molecular Biology |
Recommended Storage | Store at room temperature. |
Concentration | 1 M |
Product Line | Ambion |
Thermo Scientific™ Pierce™ Concentrated Buffer Stocks (10X and 20X)
Save time using Pierce Concentrated Buffer stock solutions (10X and 20X) with simple dilutions to expedite your experiment without sacrificing quality or consistency.
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent.
Molecular Weight (g/mol) | 238.30 |
---|---|
ChEBI | CHEBI:42334 |
Color | White |
Chemical Name or Material | HEPES |
Grade | Molecular Biology |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
ChemAlert Storage Symbol | Gray |
Assay Percent Range | ≥99 % |
PubChem CID | 23831 |
Absorbance | 0.01 max. (0.1M solution) at 280nm |
Percent Purity | ≥99% |
CAS | 7365-45-9 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
MDL Number | MFCD00006158 |
Health Hazard 2 | WARNING! |
Solubility Information | Soluble in water |
pH | 5.0 to 6.5 |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Formula | C8H18N2O4S |
Gibco™ DPBS, calcium, magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Dilution | 1 X |
---|---|
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.2 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
CAS | 10043-35-3 |
---|---|
Synonym | TBE |
Gibco™ DPBS (10X), no calcium, no magnesium
Greener Choice Product
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
This product offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More About the Greener Choice Program
Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 15°C to 30°C Shipping conditions: Room temperature Shelf life: 36 months from date of manufacture |
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Green Features | Sustainable packaging |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 2630 - 3000 mOsm/kg |
Dilution | 10 X |
Additive | No Sodium Pyruvate |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 6.7 - 7.0 |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |